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A broad panel of antibodies was employed, including those for FOXO4, p53, phospho-p53, p21, p16, cleaved-Caspase3, BAX, BCL-2, testosterone synthesis enzymes (3β-HSD, CYP11A1, CYP17A1), and various SASP factors (IL-1β, IL-6, TNF-α, TGF-β)

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We can employ several exclusion criteria to maintain a modicum of consistency among the population being tested, but no human behaves in the same manner in the presence of stressors